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SignaGen polyjet transfection reagent sl100688
Polyjet Transfection Reagent Sl100688, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+transfection+reagent+sl100688/polyjet+dna+transfection+reagent/pmc12188316-370-5-8
Average 90 stars, based on 1 article reviews
polyjet transfection reagent sl100688 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Transfection:

Article Title: Glutamine modulates stress granule formation in cancer cells through core RNA-binding proteins.
Article Snippet: Cells were transfected with a MYC binding site plasmid, containing 4 E-box regions (100 ng) and 50 ng of Renilla Luciferase pLR-TK vector (Promega). .. Transfection was performed using the PolyJet transfection reagent (SignaGen, SL100688) at 3:1 PolyJet : DNA ratio. .. Luciferase activity Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t was measured after 24 hrs using the Dual-Glo® Luciferase Assay System according to the manufacturer's instructions (Promega, E2980).

Article Title: Pharmacological Inhibition of Astrocytic Transglutaminase 2 Facilitates the Expression of a Neurosupportive Astrocyte Reactive Phenotype in Association with Increased Histone Acetylation
Article Snippet: ImageJ Fiji (version 2.14.0/1.54f) was used to quantify the intensity of each band, and all values were normalized to GAPDH or beta tubulin levels. .. For immunoprecipitation (IP), HEK 293TN was transfected with V5-TG2 and FLAG-Zbtb7a constructs using PolyJet transfection reagent (Signagen, Frederick, MD, USA, SL100688) following the manufacturer’s protocol. ..

Article Title: Subtype-specific human endogenous retrovirus K102 envelope protein is a novel serum immunosuppressive biomarker of cancer
Article Snippet: .. To produce lentiviruses, HEK293T cells in a 10-cm culture dish were transfected with a solution made of DMEM (500 μL, no FBS) together with 45 μL Polyjet transfection reagent (SignaGen, SL100688), 3 μg psPAX2 (Addgene, 12260; RRID: Addgene_12260), 3 μg pMD2.G (Addgene, 12259; RRID: Addgene_12259), and 9 μg lentiviral target plasmids (pcw-K102 Env-HA/pcw-K102 Env-Tm-HA). ..

Article Title: GAD65 tunes the functions of Best1 as a GABA receptor and a neurotransmitter conducting channel.
Article Snippet: .. Plasmid and siRNA transfections were conducted using the PolyJet Transfection Reagent (SignaGen SL100688) and Lipofectamine RNAiMAX Reagent (Invitrogen 13778-030), respectively. ..

Article Title: Glutamine modulates stress granule formation in cancer cells through core RNA-binding proteins
Article Snippet: Cells were transfected with a MYC-binding site plasmid, containing four E-box regions (100 ng) and 50 ng of Renilla Luciferase pLR-TK vector (Promega). .. Transfection was performed using the PolyJet transfection reagent (SignaGen, SL100688) at 3:1 PolyJet: DNA ratio. .. Luciferase activity was measured after 24 h using the Dual-Glo ® Luciferase Assay System according to the manufacturer's instructions (Promega, E2980).

Article Title: Ginsenoside Rb3 represses CPZ-induced demyelination and neuroinflammation by inhibiting TRAF6 K63 ubiquitination.
Article Snippet: Multiple sclerosis is a chronic inflammatory and neurodegenerative disorder of the central nervous system.. Despite ongoing research, effective treatments remain limited, especially during progressive phase.. Saponins extracted from the stem and leaf of Panax notoginseng (PNSL) demonstrate a superior anti-inflammatory effect by inhibiting NO production in LPS-induced BV2 cells.

Article Title: Curcumin targets CXCL16-mediated podocyte injury and lipid accumulation in diabetic kidney disease treatment.
Article Snippet: Among the complications of diabetes, diabetic kidney disease (DKD) frequently emerges, typified by the detrimental effects on renal function, manifesting through inflammation, dysregulated lipid metabolism, and harm to podocytes.. Existing research underscores the significance of the soluble form of C-X-C chemokine ligand 16 (CXCL16) within the context of renal impairments.. However, whether CXCL16 is involved in the pathogenesis of DKD remains elusive.

Article Title: Proteomic analysis reveals a PLK1-dependent G2/M degradation program and a role for AKAP2 in coordinating the mitotic cytoskeleton
Article Snippet: Protein A Agarose Beads , MilliporeSigma , 16-125. .. PolyJet Transfection Reagent , SignaGen , SL100688. .. DMEM, high glucose, pyruvate , Thermo Fisher Scientific , 11995065.

Immunoprecipitation:

Article Title: Pharmacological Inhibition of Astrocytic Transglutaminase 2 Facilitates the Expression of a Neurosupportive Astrocyte Reactive Phenotype in Association with Increased Histone Acetylation
Article Snippet: ImageJ Fiji (version 2.14.0/1.54f) was used to quantify the intensity of each band, and all values were normalized to GAPDH or beta tubulin levels. .. For immunoprecipitation (IP), HEK 293TN was transfected with V5-TG2 and FLAG-Zbtb7a constructs using PolyJet transfection reagent (Signagen, Frederick, MD, USA, SL100688) following the manufacturer’s protocol. ..

Construct:

Article Title: Pharmacological Inhibition of Astrocytic Transglutaminase 2 Facilitates the Expression of a Neurosupportive Astrocyte Reactive Phenotype in Association with Increased Histone Acetylation
Article Snippet: ImageJ Fiji (version 2.14.0/1.54f) was used to quantify the intensity of each band, and all values were normalized to GAPDH or beta tubulin levels. .. For immunoprecipitation (IP), HEK 293TN was transfected with V5-TG2 and FLAG-Zbtb7a constructs using PolyJet transfection reagent (Signagen, Frederick, MD, USA, SL100688) following the manufacturer’s protocol. ..

Plasmid Preparation:

Article Title: GAD65 tunes the functions of Best1 as a GABA receptor and a neurotransmitter conducting channel.
Article Snippet: .. Plasmid and siRNA transfections were conducted using the PolyJet Transfection Reagent (SignaGen SL100688) and Lipofectamine RNAiMAX Reagent (Invitrogen 13778-030), respectively. ..

Article Title: Curcumin targets CXCL16-mediated podocyte injury and lipid accumulation in diabetic kidney disease treatment.
Article Snippet: Among the complications of diabetes, diabetic kidney disease (DKD) frequently emerges, typified by the detrimental effects on renal function, manifesting through inflammation, dysregulated lipid metabolism, and harm to podocytes.. Existing research underscores the significance of the soluble form of C-X-C chemokine ligand 16 (CXCL16) within the context of renal impairments.. However, whether CXCL16 is involved in the pathogenesis of DKD remains elusive.

Luciferase:

Article Title: Ginsenoside Rb3 represses CPZ-induced demyelination and neuroinflammation by inhibiting TRAF6 K63 ubiquitination.
Article Snippet: Multiple sclerosis is a chronic inflammatory and neurodegenerative disorder of the central nervous system.. Despite ongoing research, effective treatments remain limited, especially during progressive phase.. Saponins extracted from the stem and leaf of Panax notoginseng (PNSL) demonstrate a superior anti-inflammatory effect by inhibiting NO production in LPS-induced BV2 cells.

Negative Control:

Article Title: Curcumin targets CXCL16-mediated podocyte injury and lipid accumulation in diabetic kidney disease treatment.
Article Snippet: Among the complications of diabetes, diabetic kidney disease (DKD) frequently emerges, typified by the detrimental effects on renal function, manifesting through inflammation, dysregulated lipid metabolism, and harm to podocytes.. Existing research underscores the significance of the soluble form of C-X-C chemokine ligand 16 (CXCL16) within the context of renal impairments.. However, whether CXCL16 is involved in the pathogenesis of DKD remains elusive.



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Overexpression of type 2 MUTYH ameliorates cisplatin‐induced apoptosis, <t>DNA</t> damage, and mitochondrial dysfunction in vitro. a) After <t>transfection</t> of mPTCs with type 1 or 2 human MUTYH, subcellular localization of human MUTYH were determined by immunofluorescence staining for anti‐FLAG. FLAG tag was added at the C‐terminus of the MUTYH protein. (Green: FLAG, red: Mitotracker, blue: DAPI; magnification: 630×, scale bar: 10 µm). b) Flow cytometry analysis of annexin V and PI staining and quantification of apoptosis in cisplatin‐treated (24 h) mPTCs after overexpression of type 1 or 2 human MUTYH. c) The protein levels of MUTYH and cleaved caspase3 in mPTCs overexpressing type 1 or 2 MUTYH induced by cisplatin were analyzed by Western blot. The graph on the right displays the results of the densitometry analysis. d) Representative images of the comet tail moment induced by cisplatin after type 1 or 2 human MUTYH overexpression in mPTCs. e) Fluorescent images of TMRM in MUTYH overexpressing mPTCs treated with cisplatin for 24 h. f) OCR in type 1 or type 2 MUTYH overexpression mPTCs measured using a Seahorse 96 XF‐analyzer. Cell experiments were performed three times, and data are expressed as mean ± S.D. ( n = 3). ctrl: control, Cis: cisplatin; ***P < 0.001, **P < 0.01, *P < 0.05 (one‐ or two‐way ANOVA).
Polyjet Dna Transfection Reagent Sl100688, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyjet+transfection+reagent+sl100688/polyjet+dna+transfection+reagent/pmc11967787-229-13-17
Average 90 stars, based on 1 article reviews
polyjet dna transfection reagent sl100688 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Overexpression of type 2 MUTYH ameliorates cisplatin‐induced apoptosis, DNA damage, and mitochondrial dysfunction in vitro. a) After transfection of mPTCs with type 1 or 2 human MUTYH, subcellular localization of human MUTYH were determined by immunofluorescence staining for anti‐FLAG. FLAG tag was added at the C‐terminus of the MUTYH protein. (Green: FLAG, red: Mitotracker, blue: DAPI; magnification: 630×, scale bar: 10 µm). b) Flow cytometry analysis of annexin V and PI staining and quantification of apoptosis in cisplatin‐treated (24 h) mPTCs after overexpression of type 1 or 2 human MUTYH. c) The protein levels of MUTYH and cleaved caspase3 in mPTCs overexpressing type 1 or 2 MUTYH induced by cisplatin were analyzed by Western blot. The graph on the right displays the results of the densitometry analysis. d) Representative images of the comet tail moment induced by cisplatin after type 1 or 2 human MUTYH overexpression in mPTCs. e) Fluorescent images of TMRM in MUTYH overexpressing mPTCs treated with cisplatin for 24 h. f) OCR in type 1 or type 2 MUTYH overexpression mPTCs measured using a Seahorse 96 XF‐analyzer. Cell experiments were performed three times, and data are expressed as mean ± S.D. ( n = 3). ctrl: control, Cis: cisplatin; ***P < 0.001, **P < 0.01, *P < 0.05 (one‐ or two‐way ANOVA).

Journal: Advanced Science

Article Title: HUWE1‐Mediated Degradation of MUTYH Facilitates DNA Damage and Mitochondrial Dysfunction to Promote Acute Kidney Injury

doi: 10.1002/advs.202412250

Figure Lengend Snippet: Overexpression of type 2 MUTYH ameliorates cisplatin‐induced apoptosis, DNA damage, and mitochondrial dysfunction in vitro. a) After transfection of mPTCs with type 1 or 2 human MUTYH, subcellular localization of human MUTYH were determined by immunofluorescence staining for anti‐FLAG. FLAG tag was added at the C‐terminus of the MUTYH protein. (Green: FLAG, red: Mitotracker, blue: DAPI; magnification: 630×, scale bar: 10 µm). b) Flow cytometry analysis of annexin V and PI staining and quantification of apoptosis in cisplatin‐treated (24 h) mPTCs after overexpression of type 1 or 2 human MUTYH. c) The protein levels of MUTYH and cleaved caspase3 in mPTCs overexpressing type 1 or 2 MUTYH induced by cisplatin were analyzed by Western blot. The graph on the right displays the results of the densitometry analysis. d) Representative images of the comet tail moment induced by cisplatin after type 1 or 2 human MUTYH overexpression in mPTCs. e) Fluorescent images of TMRM in MUTYH overexpressing mPTCs treated with cisplatin for 24 h. f) OCR in type 1 or type 2 MUTYH overexpression mPTCs measured using a Seahorse 96 XF‐analyzer. Cell experiments were performed three times, and data are expressed as mean ± S.D. ( n = 3). ctrl: control, Cis: cisplatin; ***P < 0.001, **P < 0.01, *P < 0.05 (one‐ or two‐way ANOVA).

Article Snippet: The sequenced CRISPR/Cas9 plasmids targeting MUTYH or HUWE1 were transfected into mPTCs with PolyJet DNA transfection reagent (SignaGen, SL100688), and positive cells were selected using puromycin (2 μg mL −1 ) for 3 days prior to clonal expansion.

Techniques: Over Expression, In Vitro, Transfection, Immunofluorescence, Staining, FLAG-tag, Flow Cytometry, Western Blot, Control